dr Mateusz Wilamowski dr Mateusz Wilamowski

Department of General Biochemistry

Faculty of Biochemistry, Biophysics and Biotechnology

Jagiellonian University

ul. Gronostajowa 7

30-387 Kraków

 

Room: C145

Phone: 12 664 62 18

E–mail: mateusz.wilamowski@uj.edu.pl

 

Education:

2013 – Mar 1st 2019  Doctorate in Biochemistry at the Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University

2008 - 2013               Graduate of Biophysics at the Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University

Employment:

Oct. 2020 – present           Adjunct at the Faculty of Biochemistry, Biophysics and Biotechnology of the Jagiellonian University

Jun 2019 – Oct. 2020       Postdoctoral scholar in the Department of Biochemistry and Molecular Biology at the University of Chicago

(PI: Prof. Andrzej Joachimiak)

Jun 2019 – Oct. 2020       Resident Associate in the Advance Protein Characterization Facility at the Argonne National Laboratory

Jan 2019 – May 2019       Teaching Assistant in the Laboratory of General Biochemistry at the Jagiellonian University 

Oct 2017 – Dec 2018        Scientific Assistant in the Laboratory of General Biochemistry at the Jagiellonian University

 

Subject of interest:

Macromolecular crystallography, serial synchrotron crystallography, time-resolved crystallography, recombinant protein expression and purification, protein chromatography, fluorescence spectroscopy, circular dichroism, molecular cloning, qPCR, western blot, immunoprecipitation.

 

Publications from last 5 years:

  • Wilamowski, M., Sherrell, D.A., Kim, Y., Lavens, A., Henning, R. W., Lazarski, K., Shigemoto, A., Endres, M., Maltseva, N., Babnigg, G., Burdette, S. C., Srajer, V., & Joachimiak, A. (2022). Time-resolved β-lactam cleavage by L1 metallo-β-lactamase. Nature Communications, 13(1), 7379
  • Wilamowski, M., Sherrell, D.A., Minasov, G., Kim, Y., Shuvalova, L., Lavens, A., Chard, R.,  Maltseva, N., Jedrzejczak, R.,  Rosas-Lemus, M., Saint, N.,  Foster, I.T., Michalska, K.,  Satchell, K.J.F, Joachimiak, A. (2021) 2′-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography. Proceedings of the National Academy of Sciences of the United States of America, 118 (21) e2100170118.
  • Sherrell, D. A., Lavens, A., Wilamowski, M., Kim, Y., Chard, R., Lazarski, K., Rosenbaum, G., Vescovi, R., Johnson, J. L., Akins, C., Chang, C., Michalska, K., Babnigg, G., Foster, I.,  Joachimiak, A. (2022). Fixed-target serial crystallography at the Structural Biology Center. Journal of Synchrotron Radiation, 29(5), 1141–1151. 
  • Wilamowski, M., Hammel, M., Leite, W., Zhang, Q., Kim, Y., Weiss, K.L., Jedrzejczak, R., Rosenberg, D.J., Fan, Y., Wower, J., Bierma, J.C., Sarker, A.H., Tsutakawa, S.E., Pingali, S.V., O'Neill, H.M., Joachimiak, A., Hura, G.L. (2021) Transient and stabilized complexes of Nsp7, Nsp8, and Nsp12 in SARS-CoV-2 replication. Biophysical Journal, 120(15), 3152 – 3165.
  • Kim, Y., Wower, J., Maltseva, N., Chang, C., Jedrzejczak, R., Wilamowski, M., Kang, S., Nicolaescu, V., Randall, G., Michalska, K., Joachimiak, A. (2021) Tipiracil binds to uridine site and inhibits Nsp15 endoribonuclease NendoU from SARS-CoV-2. Nature Communications Biology, 4(1), 193.
  • Hodge, C.D., Rosenberg, D.J., Grob, P., Wilamowski, M., Joachimiak, A., Hura, G.L.,Hammel, M. (2021) Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein. mAbs, 13(1), 1905978.
  • Dobosz, E., Wadowska, M., Kaminska, M., Wilamowski, M., Honarpisheh, M., Bryzek, D., Potempa, J., Jura, J., Lech, M., Koziel, J. (2021) MCPIP-1 Restricts Inflammation via Promoting Apoptosis of Neutrophils. Frontiers in Immunology, 12, 627922.
  • Gasiorek, A., Dobosz, E., Potempa, B., Ciaston, I., Wilamowski, M., Oruba, Z., Lamont, R., Jura, J., Potempa, J., Koziel, J. (2021) Subversion of LPS signaling in gingival keratinocytes via MCPIP-1 degradation as a novel pathogenic strategy of inflammophilic pathobionts, mBio
  • Kim, Y., Jedrzejczak, R., Maltseva, N.I., Wilamowski, M., Endres, M., Godzik, A., Michalska, K., Joachimiak, A. (2020) Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. Protein Science, 29(7), 1596-1605.
  • Pajdzik, K., Wilamowski, M., Żurawek, D., Stopa, K.B., Nodzyński, M., Kalita, A., Jura, J. (2020) Anterior gradient 2 promotes tumorigenesis through upregulation of CCAAT‐enhancer binding protein beta and hypoxia‐inducible factor‐2α and subsequent secretion of interleukin‐6, interleukin‐8, and vascular endothelial growth factor in the Caki‐1 clear cell renal cell carcinoma cell line. IUBMB Life, 72(8), 1807-1818.
  • Kim, Y., Maltseva, N., Wilamowski, M., Tesar, C., Endres, M., Joachimiak, A. (2020) Structural and biochemical analysis of the metallo-β-lactamase L1 from emerging pathogen Stenotrophomonas maltophilia revealed the subtle but distinct di-metal scaffold for catalytic activity. Protein Science, 29(3), 723-743.
  • Wilamowski, M., Gorecki, A., Dziedzicka-Wasylewska, M., Jura, J. (2018) Substrate specificity of human MCPIP1 endoribonuclease. Scientific Reports, 8:7381, 1-14.
  • Kotlinowski, J., Bukowska-Strakova, K., Koppolu, A., Kosińska, J., Pydyn, N., Stawinski, P., Wilamowski, M., Nowak, W., Józkowicz, A., Baran,J., Płoski,R., Jura,J. (2019) A novel monoallelic truncating mutation in the NFKB2 gene associated with subclinical common variable immunodeficiency. Frontiers in Genetics, 10, 140.
  • Lichawska-Cieslar, A., Pietrzycka, R., Ligeza, J., Kulecka, M., Paziewska, A., Kalita, A., Dolicka, D., Wilamowski, M., Miekus, K., Ostrowski, J., Mikula, M., Jura, J. (2018) RNA sequencing reveals widespread transcriptome changes in a renal carcinoma cell line. Oncotarget, 9, 8597–8613.
  • Lipert, B.*, Wilamowski, M.*, Gorecki, A., Jura, J. (2017) MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ. PLoS One, 12:3, 1-14.

 

Presentations at Scientific Meetings:

  • Wilamowski, M. et al. Time-resolved Cleavage of β-lactam Antibiotic by Metallo β-lactamase from S. maltophilia. EMBO Workshop - Time-resolved spectroscopy meets time-resolved crystallography: The future of dynamic photobiology, Heidelberg 2023
  • Wilamowski, M. et al. Time-Resolved Snapshots of Antibiotic Cleavage by L1 Metallo-β-Lactamase, APS users meeting, Argonne National Laboratory, USA 2021
  • Wilamowski, M. et al. Pink Beam Serial Crystallography Revealed Enzymatic States of L1 Metallo-β-Lactamase During Moxalactam Hydrolysis, BioCARS Seminars, Chicago, USA 2021
  • Wilamowski, M. et al. Serial Crystallography Reveals Cap Methylation in SARS-CoV-2, The Joint Meeting of the Polish Synchrotron Radiation Society and SOLARIS Users, Krakow, Poland 2020
  • Wilamowski, M. et al. Methylation of viral mRNA Cap by Nsp10/16 2′O MTase from SARSCoV-2, XLVIII Winter School From Proton to Proteome, Krakow, Poland 2021
  • Wilamowski, M. et al. Progress on MCPIP1 endoribonuclease, Argonne National Laboratory, USA 2018